Molecular Diversity of Alpha Toxin Produced by Strains Causing Avian Necrotic Enteritis
نویسندگان
چکیده
Nine Clostridium perfringens strains were isolated from fifteen intestinal content samples collected from commercial broilers which suffered from necrotic enteritis NE. Molecular typing of C. perfringens was performed by PCR using three sets of primers specific for toxin -producing genes of C. perfringens [alpha (900bp), beta (236bp) and epsilon (541bp)]. Six strains of C. perfringens type A and three strains type D were fully identified. Comparison of alpha toxin genes among C. perfringens type A and D field isolates by nucleotide sequence analysis revealed somewhat similarity between them and published toxin genes of C. perfringens in gene bank with percentage 84 and 75% respectively also dissimilarity between type A and D field isolates was recorded with percentage 54%.
منابع مشابه
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